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factor κb ligand rankl  (Novoprotein)

 
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    Structured Review

    Novoprotein factor κb ligand rankl
    Factor κb Ligand Rankl, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/factor+%CE%BAb+ligand+rankl/activator+receptor/pm41850405-114-33-38
    Average 86 stars, based on 1 article reviews
    factor κb ligand rankl - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Immune regulative GelMA&Zn 2+ /Ce 3+ -whitlockite scaffolds with continuous ions release for bone regeneration
    Article Snippet: .. The conditioned medium for the RAW264.7 cells was supplemented with receptor activator of nuclear factor-κB ligand (RANKL) (Novoprotein Scientific Co., Ltd., China) to a final concentration of 100 ng/mL. .. The conditioned medium for the RAW264.7 cells was supplemented with receptor activator of nuclear factor-κB ligand (RANKL) (Novoprotein Scientific Co., Ltd., China) to a final concentration of 100 ng/mL.

    In Vitro:

    Article Title: Caspase-1-targeting siRNA nanoparticles suppress pyroptosis to prevent force-induced root resorption.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Cell Culture:

    Article Title: Caspase-1-targeting siRNA nanoparticles suppress pyroptosis to prevent force-induced root resorption.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    Image Search Results


    Effects of tumor necrosis factor-like weak inducer of apoptosis on RANKL and OPG expression in PDLSCs and PDLSC-induced macrophage polarization. (A) RANKL (red) and OPG (green) expression in PDLSCs was detected by immunofluorescence staining. Scale bar, 200 μ m. Statistical analysis of the MOD values for (B) RANKL and (C) OPG, and (D) their ratio based on (A) (E) CD68 and CD163 expression in RAW264.7 macrophages was assessed by immunofluorescence staining. Scale bar, 200 μ m. Statistical analysis of the MOD values for (F) CD68 and (G) CD163 based on (E). Statistical analysis was performed using a one-way ANOVA. *** P<0.001; **** P<0.0001. Data are presented as the mean ± SD (n=5). MOD, mean optical density; ns, not significant; OPG, osteoprotegerin; PDLSC, periodontal ligament stem cell; RANKL, receptor activator of nuclear factor-κB ligand.

    Journal: International Journal of Molecular Medicine

    Article Title: TWEAK modulates the characteristics of periodontal ligament stem cells via the Fn14/NF-κB pathway

    doi: 10.3892/ijmm.2025.5679

    Figure Lengend Snippet: Effects of tumor necrosis factor-like weak inducer of apoptosis on RANKL and OPG expression in PDLSCs and PDLSC-induced macrophage polarization. (A) RANKL (red) and OPG (green) expression in PDLSCs was detected by immunofluorescence staining. Scale bar, 200 μ m. Statistical analysis of the MOD values for (B) RANKL and (C) OPG, and (D) their ratio based on (A) (E) CD68 and CD163 expression in RAW264.7 macrophages was assessed by immunofluorescence staining. Scale bar, 200 μ m. Statistical analysis of the MOD values for (F) CD68 and (G) CD163 based on (E). Statistical analysis was performed using a one-way ANOVA. *** P<0.001; **** P<0.0001. Data are presented as the mean ± SD (n=5). MOD, mean optical density; ns, not significant; OPG, osteoprotegerin; PDLSC, periodontal ligament stem cell; RANKL, receptor activator of nuclear factor-κB ligand.

    Article Snippet: The primary antibodies used for immunofluorescence staining included the mouse anti-receptor activator of nuclear factor-κB ligand (RANKL) antibody (cat. no. sc-52950; Santa Cruz Biotechnology, Inc.), rabbit anti-osteoprotegerin (OPG) antibody (cat. no. ab73400; Abcam), mouse anti-CD68 antibody (cat. no. ab201340; Abcam) and rabbit anti-CD163 antibody (cat. no. ab182422; Abcam).

    Techniques: Expressing, Immunofluorescence, Staining

    Inhibition of Fn14 and NF-κB effectively blocks TWEAK-induced alterations in the microenvironmental regulatory potential of PDLSCs. (A) Expression levels of OPG (green) and RANKL (red) in PDLSCs were detected by immunofluorescence staining, followed by quantitative analysis of the MOD values for (B) RANKL and (C) OPG, and (D) the MOD ratio of RANKL/OPG (n=5). Scale bar, 200 μ m. (E) Expression levels of CD68 and CD163 in RAW264.7 macrophages were detected by immunofluorescence staining, followed by quantitative analysis of the MOD values for (F) CD68 and (G) CD163 (n=5). Scale bar, 200 μ m. Statistical analysis was performed using a one-way ANOVA. ** P<0.01; *** P<0.001; **** P<0.0001. Data are presented as the mean ± SD. Fn14, fibroblast growth factor-inducible 14; MOD, mean optical density; ns, not significant; OPGa, osteoprotegerin; PDLSC, periodontal ligament stem cell; RANKL, receptor activator of nuclear factor-κB ligand; sh, short hairpin RNA; TWEAK, tumor necrosis factor-like weak inducer of apoptosis.

    Journal: International Journal of Molecular Medicine

    Article Title: TWEAK modulates the characteristics of periodontal ligament stem cells via the Fn14/NF-κB pathway

    doi: 10.3892/ijmm.2025.5679

    Figure Lengend Snippet: Inhibition of Fn14 and NF-κB effectively blocks TWEAK-induced alterations in the microenvironmental regulatory potential of PDLSCs. (A) Expression levels of OPG (green) and RANKL (red) in PDLSCs were detected by immunofluorescence staining, followed by quantitative analysis of the MOD values for (B) RANKL and (C) OPG, and (D) the MOD ratio of RANKL/OPG (n=5). Scale bar, 200 μ m. (E) Expression levels of CD68 and CD163 in RAW264.7 macrophages were detected by immunofluorescence staining, followed by quantitative analysis of the MOD values for (F) CD68 and (G) CD163 (n=5). Scale bar, 200 μ m. Statistical analysis was performed using a one-way ANOVA. ** P<0.01; *** P<0.001; **** P<0.0001. Data are presented as the mean ± SD. Fn14, fibroblast growth factor-inducible 14; MOD, mean optical density; ns, not significant; OPGa, osteoprotegerin; PDLSC, periodontal ligament stem cell; RANKL, receptor activator of nuclear factor-κB ligand; sh, short hairpin RNA; TWEAK, tumor necrosis factor-like weak inducer of apoptosis.

    Article Snippet: The primary antibodies used for immunofluorescence staining included the mouse anti-receptor activator of nuclear factor-κB ligand (RANKL) antibody (cat. no. sc-52950; Santa Cruz Biotechnology, Inc.), rabbit anti-osteoprotegerin (OPG) antibody (cat. no. ab73400; Abcam), mouse anti-CD68 antibody (cat. no. ab201340; Abcam) and rabbit anti-CD163 antibody (cat. no. ab182422; Abcam).

    Techniques: Inhibition, Expressing, Immunofluorescence, Staining, shRNA

    Effect of inhibition of the tumor necrosis factor-like weak inducer of apoptosis/Fn14/NF-κB/NLRP3 pathway on the microenvironmental regulatory ability of iPDLSCs. (A) Immunofluorescence staining was used to detect the expression levels of RANKL and OPG in PDLSCs, iPDLSCs, and iPDLSCs after the downregulation of Fn14, NF-κB and NLRP3, followed by statistical analysis of the average optical density values of (B) RANKL and (C) OPG, and (D) the RANKL/OPG ratio (n=5). Scale bar, 200 μ m. (E) Immunofluorescence staining was used to detect the expression levels of CD68 and CD163 in macrophages cocultured with PDLSCs, iPDLSCs, and iPDLSCs after the downregulation of Fn14, NF-κB and NLRP3, followed by statistical analysis of the average optical density values of (F) CD68 and (G) CD163 (n=5). Scale bar, 200 μ m. Statistical analysis was performed using a one-way ANOVA. * P<0.05; ** P<0.01; **** P<0.0001. Data are presented as the mean ± SD. Fn14, fibroblast growth factor-inducible 14; iPDLSC, inflammatory PDLSC; MOD, mean optical density; NLRP3, NOD-like receptor thermal protein domain-associated protein 3; ns, not significant; OPG, osteoprotegerin; PDLSC, periodontal ligament stem cell; RANKL, receptor activator of nuclear factor-κB ligand; sh, short hairpin RNA.

    Journal: International Journal of Molecular Medicine

    Article Title: TWEAK modulates the characteristics of periodontal ligament stem cells via the Fn14/NF-κB pathway

    doi: 10.3892/ijmm.2025.5679

    Figure Lengend Snippet: Effect of inhibition of the tumor necrosis factor-like weak inducer of apoptosis/Fn14/NF-κB/NLRP3 pathway on the microenvironmental regulatory ability of iPDLSCs. (A) Immunofluorescence staining was used to detect the expression levels of RANKL and OPG in PDLSCs, iPDLSCs, and iPDLSCs after the downregulation of Fn14, NF-κB and NLRP3, followed by statistical analysis of the average optical density values of (B) RANKL and (C) OPG, and (D) the RANKL/OPG ratio (n=5). Scale bar, 200 μ m. (E) Immunofluorescence staining was used to detect the expression levels of CD68 and CD163 in macrophages cocultured with PDLSCs, iPDLSCs, and iPDLSCs after the downregulation of Fn14, NF-κB and NLRP3, followed by statistical analysis of the average optical density values of (F) CD68 and (G) CD163 (n=5). Scale bar, 200 μ m. Statistical analysis was performed using a one-way ANOVA. * P<0.05; ** P<0.01; **** P<0.0001. Data are presented as the mean ± SD. Fn14, fibroblast growth factor-inducible 14; iPDLSC, inflammatory PDLSC; MOD, mean optical density; NLRP3, NOD-like receptor thermal protein domain-associated protein 3; ns, not significant; OPG, osteoprotegerin; PDLSC, periodontal ligament stem cell; RANKL, receptor activator of nuclear factor-κB ligand; sh, short hairpin RNA.

    Article Snippet: The primary antibodies used for immunofluorescence staining included the mouse anti-receptor activator of nuclear factor-κB ligand (RANKL) antibody (cat. no. sc-52950; Santa Cruz Biotechnology, Inc.), rabbit anti-osteoprotegerin (OPG) antibody (cat. no. ab73400; Abcam), mouse anti-CD68 antibody (cat. no. ab201340; Abcam) and rabbit anti-CD163 antibody (cat. no. ab182422; Abcam).

    Techniques: Inhibition, Immunofluorescence, Staining, Expressing, shRNA